Oct-3/4
Description- Octamer transcription factors 3 and 4; expressed by embryonic stem cells and germ cells; Has been reported to be expressed in germ-cell tumors and their metastases, which exhibit features of pluripotentiality, including seminoma/dysgerminoma/germinoma and embryonial carcinoma; Has been proposed as a useful marker for germ-cell tumors and to help in establishing a germ-cell origin for a few metastatic tumors of uncertain primary origin
Methodology: Immunohistochemistry
TAT: 3-5 days
Specimen Preparation
Collect - tissue /or cell
Specimen Preparation- formal fix (10 % neutral buffered formalin) and paraffin enter specimen (FFPE specimens square measure needed). shield paraffin block or slides from uncontrolled heat. Transport tissue block or five unstained (3-micron thick sections), charged slides during a tissue transport kit. (Min: a pair of slides). If causing precut slides, don't over heat it.
Storage/Transport Temperature- At temperature or cold. Transport it in cooled instrumentation throughout weather.
Unacceptable conditions- i) Specimens submitted with non-marked/tagged tissue sort square measure rejected.
ii) Depleted specimens aren't tested.
Stability- i) Ambient: Indefinitely;
ii) Refrigerated: Indefinitely;
iii) Frozen: Unacceptable
