IgH gene rearrangement

DESCRIPTION – IGH gene rearrangement is commonly found in B-cell tumors. When B-cell matures, the immunoglobin heavy chain gene is rearranged so that each mature B-cell and plasma cell has a unique rearranged heavy chain gene profile

CLINICAL UTILITY – prognostic marker for acute lymphoblastic leukemia or acute myelogenous. Covers translocations t (4;14), t (11;14) and t (14;16)

METHODOLOGY – fluorescence in situ hybridization (FISH)

TURN AROUND TIME – 8 days

PATIENT PREPARATION –

COLLECT – non diluted bone marrow aspirate and sodium heparin

SPECIMEN PREPARATION – 3-4 ml bone marrow or peripheral blood in green top or lavender top tube

STORAGE/TRANSPORT TEMPERATURE – 2-8 degrees C (with cold pack)

STABILITY – ambient: 48 hours; refrigerated: 48 hours

UNACCEPTABLE CONDITIONS – clotted specimens

MRP – 3500.00

SPECIAL INSTRUCTIONS – clinical history is mandatory