BCL6 (3q27.3) Gene Rearrangement

DESCRIPTION – BCL6 (B-cell lymphoma 6) is localized at 3q27. BCL6 rearrangement is a chromosomal abnormality in diffuse large B-cell lymphoma (DLBCL) and follicular lymphoma. Structural and microdeletions are included under structural rearrangements. FISH assay may detect the BCL6 rearrangements either in metaphase or interphase.

METHODOLOGY – florescence in situ hybridization (FISH)

TURN AROUND TIME – 12 days after sample reaches the lab

PATIENT PREPARATION –

COLLECT – tumor tissue

SPECIMEN PREPARATION – formalin fixed paraffin embedded tissue block. Protect the slides from excessive heat

STORAGE/TRANSPORT TEMPERATURE – room temperature (8-25 degrees C)

UNACCEPTABLE CONDITIONS – alternative fixatives, and decalcified specimens

STABILITY - ambient: indefinitely; refrigerated: indefinitely

MRP – 8000

SPECIAL INSTRUCTIONS – clinical history is mandatory, including surgical pathology reports