17p Deletion

DESCRIPTION – chromosome 17p deletion involves the p53 tumor suppression gene. Its product normally prevents cells having damaged DNA from progressing from the G1 to the S phase in the cell cycle.

CLINICAL UTILITY – this test is used to detect the deletion (loss of heterozygosity) of the tumor suppressor gene TP53, which can be seen in plasma cell myeloma or CLL.

METHODOLOGY – fluorescence in situ hybridization (FISH)

TURN AROUND TIME – 7 days

PATIENT PREPARATION –

COLLECT – non diluted bone marrow aspirate or sodium heparin

SPECIMEN PREPARATION – 3-4 ml bone marrow or peripheral blood in green top (sodium heparin) or lavender top (EDTA) tube

STORAGE/TRANSPORT TEMPERATURE – room temperature

STABILITY – ambient: 48 hours; refrigerated: 48 hours

UNACCEPTABLE CONDITIONS – frozen and clotted specimens

MRP – 3500.00

SPECIAL INSTRUCTIONS – clinical history is mandatory